Riccobene, Omann, Linderman, 1999

Modeling Activation and Desensitisation of G-Protein Coupled Receptors Provides Insight into Ligand Efficacy

Model Status

This CellML model has been recoded to remove the reaction element. The model is known to run in both PCEnv and COR to recreate the published results. The units have been checked and are consistent.

Model Structure

Signalling through G-protein coupled receptors is one of the most common and most important methods of transmitting information to the inside of cells. Many mathematical models have been published to describe this type of signal transduction, but the kinetic model described by Riccobene, Omann and Linderman (1999) is unique in that it includes the dynamics of G-protein activation and desensitisation, in addition to considering active and inactive receptor states (see the figure below). The model was used to predict how different ligands would affect the nature of the signal transduction response.

The complete original paper reference is cited below:

Modeling Activation and Desensitisation of G-Protein Coupled Receptors Provides Insight into Ligand Efficacy, Todd A. Riccobene, Geneva M. Omann and Jennifer J. Linderman, 1999 Journal of Theoretical Biology , 200, 207-222. (A PDF version of the article is available for Journal Members on the Journal of Theoretical Biology website.) PubMed ID: 10504286

A schematic diagram of Riccobene, Omann and Linderman's model of G-protein coupled receptor signalling. R is the inactive form of the receptor, R* is the active form of the receptor, R_ds is the desensitised form of the receptor, L represents the ligand (in this case, N-formyl-norleucyl-leucyl-phenylalaninyl-nor-leucyl-tyrosyl-lysine-fluorescein, or CHO-NLFNTK-fl), G is the inactive G-protein, G* is the activated G-protein, and LR/LR*/LR_ds represents the ligand/receptor complex in its various forms.