Single_stim_experiment.cellml

Single stimulation experiment

In the Single stimulation experiment, the Excitation-contraction component is configured and parameterised with an applied Single pulse patch clamp protocol.

You can change the parameters of stimulation in the component Vstim_para.

You can also bypass the parameters in the component model_parameters and initial_conditions and define your own parameters. We defined control_para to alter some parameters such as intracellular Na +  concentration for specific experiments.

The simulation experiment can be obtained by loading the corresponding SED-ML document into OpenCOR and executing the simulation.

Control of Intracellular Ca2 +  Concentration

The experiment setting to reproduce Figure 2-4 is summarized in the following:

table1

Experiment setting.

Figure [1] shows simulations of Ca2 + i decay (in nM). A: Ca2 + i decay under inhibition of Ca2 +  pumps. B: Ca2 + i decay in control conditions.

[1]
simFig2

Simulated Ca2 + i decay (in nM).

Figure [2] shows simulations of Ca2 + i rise and decay following a 200 ms voltage pulse from a holding potential of -50 mV to pulse potentials of 0 mV (A), 10 mV (B), and -10 mV (C).

[2]
simFig3

Simulated Ca2 + i rise and decay.

Figure [3] shows simulations of Ca2 + i rise and decay following a 200 ms voltage pulse from a holding potential of -50 mV to pulse potentials of -20 mV (A) and 20 mV (B).

[3]
simFig4

Simulated Ca2 + i rise and decay.

The experiment setting to reproduce primary Figure 5A and 5B are the same as the one used in primary Figure 2B and Figure 3A, respectively.

Figure [4] shows simulations of Ca2 +  fluxes through various Ca2 +  control mechanisms. Plot A shows Ca2 +  flux through Na +  ⁄ Ca2 +  exchangers and Ca2 +  pumps during Ca2 + i decay for a holding potential of -80 mV followed by a 750 ms voltage pulse to 0 mV, while plot B shows Ca2 +  flux through Ca2 +  channels and Ca2 +  extraction mechanisms during Ca2 +  rise and decay in response to a 200 ms voltage pulse to 0 mV from a holding potential of -50 mV.

[4]
simFig5

Simulated Ca2 +  fluxes through various Ca2 + i control mechanisms.